eLife

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching

This important study builds on previous work from the same authors to present a conceptually distinct workflow for cryo-EM reconstruction that uses 2D template matching to enable high-resolution ...
eLife

Separating selection from mutation in antibody language models

This important study introduces a new biology-informed strategy for deep learning models aiming to predict mutational effects in antibody sequences. It provides solid evidence that separating ...
Nature

Computer science articles from across Nature Portfolio

Computer science is the study and development of the protocols required for automated processing and manipulation of data. This includes, for example, creating algorithms for efficiently searching ...
Nature

Rapid and efficient site-directed mutagenesis by the single-tube megaprimer PCR method

1| In a sterile 0.5-ml microcentrifuge tube or amplification tube (on ice), mix the following reagents for the first amplification reaction: 2| Place the tubes in a thermocycler and amplify the ...